How Karyotypes Are Prepared?

Campbell Biology

A karyotype is a display of condensed chromosomes arranged in pairs. Karyotyping can be used to screen for defective chromosomes or abnormal numbers of chromosomes associated with certain congenital disorders, such as Down syndrome.

Source: Urry, Lisa A.. Campbell Biology (p. 256). Pearson Education. Kindle Edition

In the picture above, karyotypes are prepared from isolated somatic cells, which are treated with a drug to stimulate mitosis and then grown in culture for several days. Cells arrested when the chromosomes are most highly condensed—at metaphase—are stained and then viewed with a microscope equipped with a digital camera. An image of the chromosomes is displayed on a computer monitor, and digital software is used to arrange them in pairs according to their appearance.

Source: Urry, Lisa A.. Campbell Biology (p. 256). Pearson Education. Kindle Edition

The karyotype in the above picture shows the chromosomes from a human male (as seen by the presence of the XY chromosome pair), colored to emphasize their chromosome banding patterns. The size of the chromosome, position of the centromere, and pattern of stained bands help identify specific chromosomes. Although difficult to discern in the karyotype, each metaphase chromosome consists of two closely attached sister chromatids.

Source:

Urry, Lisa A.. Campbell Biology (p. 256). Pearson Education. Kindle Edition

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